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The bone marrow biopsy report showed 65% cellularity with a left shift in myeloid maturation, increased erythrocyte precursors, and decreased megakaryocytes

In short, tissues were incubated with PBS containing EDTA at 37 C for 2 20 min, then digested twice for 35 min with a cocktail of collagenase D (1 mg/ml, Roche), dispase I (0.1 mg/ml, Roche), and DNase I (333 mg/ml, Sigma), smashed and filtered through a 40 mm gauze (BD Biosciences)

Elution of the bound proteins is carried out through competition with the flag peptide, which has high affinity and specificity to anti-flag antibody [105]

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